Differences in neutralizing antigenicity between laboratory and clinical isolates of HCoV-229E isolated in Japan in 2004-2008 depend on the S1 region sequence of the spike protein.
Identifieur interne : 001D33 ( Main/Exploration ); précédent : 001D32; suivant : 001D34Differences in neutralizing antigenicity between laboratory and clinical isolates of HCoV-229E isolated in Japan in 2004-2008 depend on the S1 region sequence of the spike protein.
Auteurs : Kazuya Shirato ; Miyuki Kawase ; Oshi Watanabe [Japon] ; Chika Hirokawa [Japon] ; Shutoku Matsuyama ; Hidekazu Nishimura [Japon] ; Fumihiro Taguchi [Japon]Source :
- The Journal of general virology [ 1465-2099 ] ; 2012.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Anticorps antiviraux (immunologie), Coronavirus humain 229E (), Coronavirus humain 229E (génétique), Coronavirus humain 229E (immunologie), Coronavirus humain 229E (isolement et purification), Délétion de séquence, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Infections à coronavirus (immunologie), Infections à coronavirus (virologie), Japon, Jeune adulte, Lignée cellulaire, Motifs d'acides aminés, Mâle, Phylogénie, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Tests de neutralisation.
- MESH :
- génétique : Coronavirus humain 229E, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- immunologie : Anticorps antiviraux, Coronavirus humain 229E, Glycoprotéines membranaires, Infections à coronavirus, Protéines de l'enveloppe virale.
- isolement et purification : Coronavirus humain 229E.
- virologie : Infections à coronavirus.
- Adulte, Adulte d'âge moyen, Coronavirus humain 229E, Délétion de séquence, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Japon, Jeune adulte, Lignée cellulaire, Motifs d'acides aminés, Mâle, Phylogénie, Protéines de l'enveloppe virale, Tests de neutralisation.
- Wicri :
- geographic : Japon.
English descriptors
- KwdEn :
- Adult, Amino Acid Motifs, Antibodies, Viral (immunology), Cell Line, Coronavirus 229E, Human (classification), Coronavirus 229E, Human (genetics), Coronavirus 229E, Human (immunology), Coronavirus 229E, Human (isolation & purification), Coronavirus Infections (immunology), Coronavirus Infections (virology), Female, Humans, Japan, Male, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Middle Aged, Neutralization Tests, Phylogeny, Sequence Deletion, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Young Adult.
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- geographic : Japan, Spike Glycoprotein, Coronavirus.
- classification : Coronavirus 229E, Human.
- genetics : Coronavirus 229E, Human.
- immunology : Coronavirus 229E, Human, Coronavirus Infections.
- isolation & purification : Coronavirus 229E, Human.
- virology : Coronavirus Infections.
- Adult, Amino Acid Motifs, Cell Line, Female, Humans, Male, Middle Aged, Neutralization Tests, Phylogeny, Sequence Deletion, Young Adult.
Abstract
Human coronavirus (HCoV) is a causative agent of the common cold. Although HCoV is highly prevalent in the world, studies of the genomic and antigenic details of circulating HCoV strains have been limited. In this study, we compared four Japanese isolates with the standard HCoV-229E strain obtained from ATCC (ATCC-VR740) by focusing on the spike (S) protein, a major determinant of neutralizing antigen and pathogenicity. The isolates were found to have nucleotide deletions and a number of sequence differences in the S1 region of the S protein. We compared two of the Japanese isolates with the ATCC-VR740 strain by using virus-neutralizing assays consisting of infectious HCoV-229E particles and vesicular stomatitis virus (VSV)-pseudotyped virus carrying the HCoV-229E S protein. The two clinical isolates (Sendai-H/1121/04 and Niigata/01/08) did not react with antiserum to the ATCC-VR740 strain via the neutralizing test. We then constructed a pseudotype VSV-harboured chimeric S protein with the ATCC S1 and Sendai S2 regions or that with Sendai S1 and ATCC S2 regions and compared them by a neutralization test. The results revealed that the difference in the neutralizing antigenicity depends on the S1 region. This different antigenic phenotype was also confirmed by a neutralizing test with clinically isolated human sera. These results suggest that the HCoV-229E viruses prevalent in Japan are quite different from the laboratory strain ATCC-VR740 in terms of the S sequence and neutralization antigenicity, which is attributed to the difference in the S1 region.
DOI: 10.1099/vir.0.043117-0
PubMed: 22673931
Affiliations:
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Le document en format XML
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Department of Virology III, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011 Japan.</nlm:affiliation><wicri:noCountry code="subField">Tokyo 208-0011 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Watanabe, Oshi" sort="Watanabe, Oshi" uniqKey="Watanabe O" first="Oshi" last="Watanabe">Oshi Watanabe</name><affiliation wicri:level="1"><nlm:affiliation>Virus Research Center, Sendai Medical Center, Sendai, Miyagi 983-8520, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Virus Research Center, Sendai Medical Center, Sendai, Miyagi 983-8520</wicri:regionArea><wicri:noRegion>Miyagi 983-8520</wicri:noRegion></affiliation></author><author><name sortKey="Hirokawa, Chika" sort="Hirokawa, Chika" uniqKey="Hirokawa C" first="Chika" last="Hirokawa">Chika Hirokawa</name><affiliation wicri:level="1"><nlm:affiliation>Virology Section, Niigata Prefectural Institute of Public Health and Environmental Sciences, Niigata 950-2144, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Virology Section, Niigata Prefectural Institute of Public Health and Environmental Sciences, Niigata 950-2144</wicri:regionArea><wicri:noRegion>Niigata 950-2144</wicri:noRegion></affiliation></author><author><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name><affiliation><nlm:affiliation>Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011 Japan.</nlm:affiliation><wicri:noCountry code="subField">Tokyo 208-0011 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Nishimura, Hidekazu" sort="Nishimura, Hidekazu" uniqKey="Nishimura H" first="Hidekazu" last="Nishimura">Hidekazu Nishimura</name><affiliation wicri:level="1"><nlm:affiliation>Virus Research Center, Sendai Medical Center, Sendai, Miyagi 983-8520, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Virus Research Center, Sendai Medical Center, Sendai, Miyagi 983-8520</wicri:regionArea><wicri:noRegion>Miyagi 983-8520</wicri:noRegion></affiliation></author><author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de 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Aged</term><term>Neutralization Tests</term><term>Phylogeny</term><term>Sequence Deletion</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Anticorps antiviraux (immunologie)</term><term>Coronavirus humain 229E ()</term><term>Coronavirus humain 229E (génétique)</term><term>Coronavirus humain 229E (immunologie)</term><term>Coronavirus humain 229E (isolement et purification)</term><term>Délétion de séquence</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Infections à coronavirus (immunologie)</term><term>Infections à coronavirus (virologie)</term><term>Japon</term><term>Jeune adulte</term><term>Lignée cellulaire</term><term>Motifs d'acides aminés</term><term>Mâle</term><term>Phylogénie</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Tests de neutralisation</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Japan</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus 229E, Human</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus 229E, Human</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus humain 229E</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Coronavirus humain 229E</term><term>Glycoprotéines membranaires</term><term>Infections à coronavirus</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus 229E, Human</term><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus 229E, Human</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus humain 229E</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Amino Acid Motifs</term><term>Cell Line</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Neutralization Tests</term><term>Phylogeny</term><term>Sequence Deletion</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Coronavirus humain 229E</term><term>Délétion de séquence</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Japon</term><term>Jeune adulte</term><term>Lignée cellulaire</term><term>Motifs d'acides aminés</term><term>Mâle</term><term>Phylogénie</term><term>Protéines de l'enveloppe virale</term><term>Tests de neutralisation</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Japon</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human coronavirus (HCoV) is a causative agent of the common cold. Although HCoV is highly prevalent in the world, studies of the genomic and antigenic details of circulating HCoV strains have been limited. In this study, we compared four Japanese isolates with the standard HCoV-229E strain obtained from ATCC (ATCC-VR740) by focusing on the spike (S) protein, a major determinant of neutralizing antigen and pathogenicity. The isolates were found to have nucleotide deletions and a number of sequence differences in the S1 region of the S protein. We compared two of the Japanese isolates with the ATCC-VR740 strain by using virus-neutralizing assays consisting of infectious HCoV-229E particles and vesicular stomatitis virus (VSV)-pseudotyped virus carrying the HCoV-229E S protein. The two clinical isolates (Sendai-H/1121/04 and Niigata/01/08) did not react with antiserum to the ATCC-VR740 strain via the neutralizing test. We then constructed a pseudotype VSV-harboured chimeric S protein with the ATCC S1 and Sendai S2 regions or that with Sendai S1 and ATCC S2 regions and compared them by a neutralization test. The results revealed that the difference in the neutralizing antigenicity depends on the S1 region. This different antigenic phenotype was also confirmed by a neutralizing test with clinically isolated human sera. These results suggest that the HCoV-229E viruses prevalent in Japan are quite different from the laboratory strain ATCC-VR740 in terms of the S sequence and neutralization antigenicity, which is attributed to the difference in the S1 region.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Kawase, Miyuki" sort="Kawase, Miyuki" uniqKey="Kawase M" first="Miyuki" last="Kawase">Miyuki Kawase</name><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name></noCountry><country name="Japon"><noRegion><name sortKey="Watanabe, Oshi" sort="Watanabe, Oshi" uniqKey="Watanabe O" first="Oshi" last="Watanabe">Oshi Watanabe</name></noRegion><name sortKey="Hirokawa, Chika" sort="Hirokawa, Chika" uniqKey="Hirokawa C" first="Chika" last="Hirokawa">Chika Hirokawa</name><name sortKey="Nishimura, Hidekazu" sort="Nishimura, Hidekazu" uniqKey="Nishimura H" first="Hidekazu" last="Nishimura">Hidekazu Nishimura</name><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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